Fig 5. scMAYV-E induces both CD4+ and CD8+ T cell responses in mice.

Splenocytes from immunized mice as outlined in the experiments from Fig 5 were also evaluated by polychromatic flow cytometry to identify the frequency of (A) CD4⁺ and (B) CD8⁺ T cells that produce the cytokines IFN-γ, IL-2, and TNF-α following a 5 hour ex vivo stimulation with pooled MAYV envelope peptides spanning the entire length of the envelope protein. One representative experiment of three is shown in A and B. (C) Frequency of total CD4⁺ and CD8⁺ T cells expressing each of the seven analyzed combinations of IFN-γ, TNF-α, and IL-2 using Boolean gating is shown as bar graphs. The pie charts represent the proportion of CD4+ and CD8+ T cells producing one, two, or all three cytokines.