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Fluorescence emission spectra of a 1.0 μM solution of MagQ1 treated with increasing concentrations of Mg2+. Titration conducted in 50 mM aqueous PIPES buffer, 100 mM KCl, pH 7.0, 25 °C. Excitation wavelength λex = 600 nm. Inset: non-linear fit (red line) of the integrated fluorescence emission (black circles) as a function of total magnesium concentration, [Mg2+]t, using a 1:1 metal-to-indicator binding model.
