Table 1.
Compound | λabs (nm) |
λexc (nm) |
λem (nm) |
Φb | Kd Mg2+ | Fmax/Fmin |
---|---|---|---|---|---|---|
MagQ1c | 600e | 600 | 635 | 0.0108(3) | ||
MagQ1 + Mg2+d | 602 | 602 | 636 | 0.35(1) | 1.50(2) mM | 29 |
MagQ2c | 600 | 600 | 634 | 0.0099(8) | ||
MagQ2 + Mg2+d | 601 | 601 | 635 | 0.34(2) | 1.51(5) mM | 29 |
Data collected at a probe concentration of 1 μM in aqueous buffer (50 mM PIPES, 100 mM KCl, pH 7.0, 25 °C). Excitation wavelength λexc = 600 nm. Number in parenthesis correspond to uncertainty in the last significant figure
Relative quantum yield of fluorescence was determined using a cresyl violet solution in methanol as fluorescence
standard (Φ = 0.66).
Solution treated with 10 μM EDTA to remove adventitious metal.
Solution containing 200 mM Mg2+.
Molar absorptivity ε = 84000 ± 1000 M−1⋅cm−1.