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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: J Mol Cell Cardiol. 2018 Oct 22;125:140–148. doi: 10.1016/j.yjmcc.2018.10.009

Fig. 7. Myosin cosedimentation assays: NTE chimeric mutants.

Fig. 7.

Myosin cosedimentation assays comparing mouse (white), human (gray) and N-terminal variant C0-C2 proteins of short form (no NTE) mouse C0-C2 (mSF2), long form (with the mouse NTE) human C0-C2 (hLF), and N-terminal tagged human C0-C2 (hNT) control. Mole fraction (relative to myosin) binding was determined for all forms at 40 μM C0-C2 and 0.78 μM mouse cardiac myosin. Values were normalized to the level of cosedimentation observed for human C0-C2 (see Methods). *p < 0.01, **p < 0.005, and ***p < 0.0001 indicate a significant difference as compared to wild type human C0-C2.