Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Yeast: Saccharomyces cerevisiae) |
W303 | SGD:https://www.yeastgenome.org/strain/S000203491 | KWY XYZ | MATa/MATα {leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15} [phi+] |
| Strain, strain background (Escherichia coli) |
E.Coli DH5α | Thermo Fisher Scientific |
18258012 | |
| Strain, strain background (Escherichia coli) |
E.Coli BL21 star (DE3) | Thermo Fisher Scientific |
C601003 | |
| Genetic reagent () |
Please see Supplementary file 1, 2—S2A |
|||
| Antibody | mouse-anti-GFP | Roche | Cat# 11814460001, RRID: AB_390913 | Western blot: 1:1000 |
| Antibody | rabbit-anti-Hxk1 | US Biological | Cat# H2035-01, RRID: AB_2629457, Salem, MA | Western blot: 1:3000 |
| Antibody | IRdye 680RD goat-anti-rabbit |
LI-COR Biosciences | Cat# 926–68071, RRID: AB_10956166 | Western blot: 1:5000 |
| Antibody | IRdye 800 donkey-anti-mouse |
LI-COR Biosciences | Cat# 926–32212, RRID: AB_621847 | Western blot: 1:10000 |
| Antibody | Rabbit-anti-Dhh1 | in house | #100 | Western blot: 1:5000 |
| Recombinant DNA reagent |
Please see Supplementary file 2—table S2B | |||
| Chemical compound, drug |
hippuristanol | kind gift from Junichi Tanaka, University of the Ryukyus |
hippuristanol | 10 µM in DMSO |
| Chemical compound, drug |
cyclohexi | Sigma-Aldrich, CH | CAS Number 66-81-9 | 50 ug/ml in DMSO |
| Software, algorithm |
Diatrack | http://www.diatrack.org/ | Diatrack | used for counting PB number and intensity |
| Software | fiji/imagej | NIH | https://fiji.sc/ | adjusting brightness, contrast and making final figures |