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. 2018 Nov 30;211(2):419–430. doi: 10.1534/genetics.118.301705

Figure 4.

Figure 4

The GR-LBD adduct modulates protein activity in vivo. (A) Representative fluorescent micrographs of L1 transgenic animals expressing the GR LBD fused in frame to the N terminus of GFP and under control of the heat shock-inducible hsp-16.48 promoter. Animals were heat shocked followed by treatment with vehicle or 100 μM dex for a total of 4 hr. Inset boxes highlight zoomed-in regions near the midbody of the animals; ▵’s denote cytoplasmic and nuclear expression of GFP. Pharyngeal fluorescence is from the co-injection marker myo-2p::tdTomato. Bottom bars represent the percentage of mixed staged, tdTomato-expressing animals with either nuclear only (shaded) or cytoplasmic and nuclear expression of GFP after 4 hr of vehicle or dex treatment (open). * P < 0.00001, chi-square test. Bar, 10 μm. (B) The relative transcript levels of the DAF-16 target gene, sod-3, after heat shock and ligand treatment for 4 hr in daf-2(e1370) mutants and wild-type animals carrying a GR LBD::DAF-16A transgene. The graph depicts the mean fold change of expression, relative to vehicle-treated N2 animals lacking any transgenes. Error bars indicate the SEM from at least four biological replicates performed in triplicate. * P < 0.03, t-test.