Figure 2.

DOT6 size epistasis. Evaluation of size epistasis between dot6 and different potent Start mutations. DOT6 was inactivated in sch9 (A), sfp1 (B), hog1 (C), and swi4 (D) mutants, and the resulted double mutant strains were analyzed for cell size distribution. (E) SWI4 deletion is epistatic to DOT6 regarding the growth rate. Cells were grown in YPD medium at 30° under agitation, and cells were counted using the Z2-Coulter Counter. Results are the mean of three replicates. (F) Summary of DOT6 genetic interactions with the C. albicans Start machinery.