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. 2018 Dec 13;211(2):531–547. doi: 10.1534/genetics.118.301727

Figure 6.

Figure 6

Dam1p S31 phosphorylation is regulated by glucose and cAMP. (A) Overnight cultures of Dam1p-HBH (JCY986) were grown in YP 2% glucose. Fresh cultures from the overnight were prepared in YP 2% glucose and grown until midlog phase, then split into YP 2% glucose or into YP 0.05% glucose for 4 hr. Dam1p-HBH was purified from cells and analyzed (lanes 1 and 2). Alternatively, Dam1p-HBH was purified from asynchronously grown cultures of (JCY986) in YP 0.05% glucose started from an overnight culture grown in YP 0.05% glucose (*, lane 3). (B) Dam1p-HBH was purified from cells (JCY986) arrested with α-factor. Cultures were treated with α-factor and cells were collected only when >95% cells appeared to be arrested with a shmoo morphology. Two replicates of the α-factor arrest experiment is shown.