Figure 1.

DNA methylation is abnormal in dim-1 strains at select loci, and DIM-1 has features of ATP-dependent chromatin remodeling factors. (A and B) Southern blot of genomic DNA from the indicated strains digested with either DpnII (D) or its 5^mC-sensitive isoschizomer BfuCI (B), and probed for the heterochromatic regions 8:A6 (A) or 8:G3 (B). To test for complementation of the dim-1 mutation, we crossed in a WT allele inserted at the his-3 locus (“+dim-1”) (C). Primary structure schematics of DIM-1 and its homologs from yeasts and humans with known domains are indicated, amino acid coordinates are shown below, and positions of nonsense mutations in dim-1 strains are shown by asterisks.