Figure 2.

Polymorphisms in RAD5 underlie mutation rate variation. (A) RAD5 polymorphisms between BY and RM are located in the helicase region. The first letter for each polymorphism indicates the BY polymorphisms (E783, I791) and the second letter indicates the RM polymorphisms (D783, S791). (B) The effect of single RAD5 polymorphism and RAD5 whole-gene replacement was tested in the BY strain background for mutation rate. For each strain, the mutation rates of 96 replicates were measured. Bold lines show the mean. Boxes show the interquartile range. Statistical significance was tested using a permutation t-test. Permutation P value <0.001 is shown as ***.