Figure 10.

GLD-1 requires interaction with DLC-1 to prevent ectopic germline proliferation in vivo. Gonads were immunostained for mitotically dividing cells (pink), stem and progenitor cells (green), and DNA (blue) as in Figure 2. Dissected germlines from gld-1(q485); gld-1^wt::ollas transgenic animals treated with either (A) empty vector control or (B) gld-2(RNAi). Dissected gonads from gld-1(q485); gld-1^ndb::ollas mutant worms treated with either (C) control or (D) gld-2(RNAi). Efficacy of gld-2(RNAi) was determined by scoring sterility and embryonic lethality. gld-1(q485); gld-1^wt::ollas transgenic animals fed gld-2(RNAi) exhibited 100% sterility and gld-1(q485); gld-1^ndb::ollas mutant worms exhibited on average 86% sterility and the embryonic lethality of worms with eggs ranged from 43 to 100%. White arrow indicates aberrant cell proliferation. Images represent data collected from two independent experiments and 19–132 worms were scored for each genotype (see Table 3). Bar, 10 μm. (E) Mitotic region length was measured by counting REC-8 positive stem and progenitor cell row number of gld-1(q485); gld-1^wt::ollas (blue) and gld-1(q485); gld-1^ndb::ollas (green) transgenic animals fed either control, gld-2(RNAi), or gld-3(RNAi), respectively. A total of 22–80 germlines were scored for each genotype (N, indicated below the chart). A statistically significant difference in distribution of mitotic zone lengths was observed between gld-1(q485); gld-1^wt::ollas and gld-1(q485); gld-1^ndb::ollas worms treated with gld-3(RNAi) (indicated by *; P = 0.024) but not gld-1(q485); gld-1^wt::ollas vs. gld-1(q485); gld-1^ndb::ollas worms treated with gld-2(RNAi) (P > 0.4). Kolmogorov–Smirnov test was used to calculate P-values. Embryonic lethality of gld-1(q485); gld-1^wt::ollas; gld-3(RNAi) worms was 69 ± 25% and gld-1(q485); gld-1^ndb::ollas; gld-3(RNAi) was 54 ± 11%.