Figure 3.

Length of mitotic region in dlc-1, dhc-1 and gld mutant germlines. To identify defects in the transition from mitosis to meiosis, the number of REC-8–positive stem and progenitor cell rows from the distal end of the germline to the transition zone boundary were counted. A statistically significant increase in the lengths of the distal proliferative zones was observed in gld-2(q497); dlc-1(tm3153) germlines compared to gld-2(q497) control (indicated by **; P = 0.0025 by Kolmogorov–Smirnov test). No significant changes in mitotic zone length were detected in gld-3(q730); dlc-1(tm3153) vs. gld-3(q730) germlines (P > 0.25 by Kolmogorov–Smirnov test). Data were collected from at least two independent experiments and 24–80 germlines were scored for each genotype.