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. 2018 Dec 3;211(2):665–681. doi: 10.1534/genetics.118.301617

Figure 5.

Figure 5

DLC-1 helps regulate a subset of GLD-1 target mRNAs in a dynein motor independent manner. Fluorescence micrographs of dissected gonads expressing GFP-tagged Histone H2B under the control of either (A) cye-1 3′UTR or (B) spn-4 3′UTR after the indicated RNAi treatments. Bar, 10 μm. (C) Quantitation of reporter expression in the meiotic pachytene region of C. elegans germline after the following RNAi treatments: control (gray), dlc-1 (green), gld-1 (yellow), and dhc-1 (blue). Transgenes are identified along the x-axis, and the number of germlines scored (N) is indicated for each treatment. Efficiencies of dlc-1 and dhc-1(RNAi) treatments were established by confirming 100% sterility. Nuclear GFP signal in meiotic pachytene germ cells was quantified using LAS-X software (Leica). Signal intensities following experimental RNAi treatments (dlc-1, gld-1, or dhc-1) were normalized to respective control values. Results are representative of at least three independent experiments and error bars represent SD from the mean. Student’s unpaired t-test was used to calculate P-values for dlc-1 and dhc-1(RNAi) treatments compared to control. * indicates statistically significant differences.