Figure 3.

Characterization of rhodopsin light-dependent function. (A) Response to light from yeast-expressed rhodopsin mutants, indicating a similar magnitude of response as the mammalian cell-expressed protein. WT: Wild-type human rhodopsin. Yeast data points represent results of nine individual colonies, each in a 600 μl culture, minus the mCherry fluorescence of the same strain transformed with empty plasmid control (Vector), and normalized to wild-type. * P < 0.05 vs. WT or between indicated mutants. (B) Difference spectra of mammalian cell-expressed rhodopsin mutants in response to light. The peak at 500 nm indicates a light-dependent response.