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. 2019 Feb 7;14(2):e0211937. doi: 10.1371/journal.pone.0211937

Fig 5. FTO seems to regulate BDNF processing through modulation of the MMP-9 amounts.

Fig 5

(A) Representative western blot images for proteins involved in the cleavage of BDNF showing the protein levels of PC1/3, Furin, Plasminogen, TIMP1, MMP-7 and MMP-9 in the hippocampus. Actin or GAPDH were used as loading controls. (B-G) Quantification of western blots indicating no differences in the protein amounts of PC1/3 (B), Furin (C), Plasminogen (D), TIMP1 (E) and MMP-7 (F) between genotypes (n = 3) but a decreased protein amount of MMP-9 (G) in Fto-/- mice (n = 7). (H-J) Quantification of Mmp-3 (H), Mmp-7 (I) and Mmp-9 (J) mRNA transcripts detected in the hippocampus by real-time PCR. The mRNA expression of Mmp-9 is reduced in the hippocampus of Fto-/- mice, n = 6 Fto+/+ / 7 Fto-/-. Data are presented as mean ± S.E.M., unpaired t-test, **P < 0.01 ***P < 0.001.