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. Author manuscript; available in PMC: 2019 Feb 7.
Published in final edited form as: Cell Rep. 2018 Oct 30;25(5):1255–1267.e5. doi: 10.1016/j.celrep.2018.10.023

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit polyclonal anti-TRPS1 Bethyl Cat # A303-563A; RRID: AB_10949357
Rabbit polyclonal anti-p66 alpha abcam Cat # ab87663; RRID: AB_1952305
Rabbit polyclonal anti-p66 beta Bethyl Cat # A301-283A; RRID: AB_937930
Rabbit polyclonal anti-SNF2H abcam Cat # ab3749; RRID: AB_2191856
Mouse monoclonal anti-alpha tubulin Sigma-Aldrich Cat # T9026; RRID: AB_477593
Rabbit polyclonal anti-pHistone 3 (Ser 10) Millipore Cat # 06-570; RRID: AB_310177
Rabbit polyclonal anti-MTA2 abcam Cat # ab8106; RRID: AB_306276
Mouse monoclonal anti-HDAC2 abcam Cat # ab12169; RRID: AB_2118547
Rabbit polyclonal anti-H3K27ac Diagenode Cat # C15410196; RRID: AB_2637079
Mouse monoclonal anti-H3K27me3 abcam Cat # ab6002; RRID: AB_305237
Chemicals, Peptides, and Recombinant Proteins
TransIT-293 Transfection Reagent Mirus Cat # MIR2700
Polybrene Millipore Cat # TR-1003-G
Puromycin InvivoGen Cat # ant-pr-1
Critical Commercial Assays
CellTiter-Glo Luminescent Cell Viability Assay Promega Cat # G7573
ThruPLEX DNA-seq 48S Kit Rubicon Cat # R400427
QIAamp DNA Blood Maxi kit QIAGEN Cat #51194
QIAamp DNA Mini kit QIAGEN Cat # 51304
RNeasy Mini Kit QIAGEN Cat# 74106
Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit Life Technologies Cat # V13241
Deposited Data
Raw genomic data NCBI GEO GEO: GSE114600
Experimental Models: Cell Lines
HEK293T cells ATCC Cat # CRL-11268
59 cancer cell lines used in the screen (Table S1) various N/A
HCC3153 shTRPS1-1 cells This paper N/A
HCC3153 shTRPS1-1 RFP-H2B cells This paper N/A
HCC3153 shTRPS1-2 cells This paper N/A
HCC3153 shTRPS1-2 RFP-H2B cells This paper N/A
Oligonucleotides
shTRPS1-1a: CCGGCTGAGGTCCTGACAAGCGATACTCGAGTATCGCTTGTCAGGACCTCAGTTTTT This paper N/A
shTRPS1-1b: AATTAAAAACTGAGGTCCTGACAAGCGATACTCGAGTATCGCTTGTCAGGACCTCAG This paper N/A
shTRPS1-2a: CCGGGAGGTCCTGACAAGCGATAACCTCGAGGTTATCGCTTGTCAGGACCTCTTTTT This paper N/A
shTRPS1-2b: AATTAAAAAGAGGTCCTGACAAGCGATAACCTCGAGGTTATCGCTTGTCAGGACCTC This paper N/A
Recombinant DNA
pRSI16cb-U6-sh-13kCB18-HTS6-UbiC-TagRFP-2A-Puro McDonald et al., 2017 Cellecta Cat # SVSHU616-L
13K-hTF McDonald et al., 2017 Cellecta Cat # 13K-hTF-GH-NOVA
13K-hEPI2 McDonald et al., 2017 Cellecta Cat # 13K-hEPI2-GHNOVA
Lentiviral Packaging Plasmid Mix Cellecta Cat # CPCP-K2A
Tet-pLKO-puro Wiederschain et al., 2009 Addgene Cat #21915
pBABE-RFP-H2B David Pellman (DFCI) N/A