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. Author manuscript; available in PMC: 2019 Dec 18.

Published in final edited form as: Circulation. 2018 Dec 18;138(25):2919–2930. doi: 10.1161/CIRCULATIONAHA.118.035200

Figure 1. — (A) Diagram of the Sca-1^{H2B-tdTomato/+};c-Kit^H2B-GFP/+ double heterozygous alleles. (B-C) Longitudinal sections of Sca-1^{H2B-tdTomato/+};c-Kit^H2B-GFP/+ mouse hearts at P30 (B) and P120 (C). Partial Sca-1^H2B-tdTomato cells and c-Kit^H2B-GFP cells showed co-localization (yellow, arrows in B2 and C2). (D) Flow cytometry analysis of 4-month-old Sca-1^{H2B-tdTomato/+};c-Kit^H2B-GFP/+ mouse heart cells. (E) Diagram of the Sca-1^{H2B-tdTomato/+};PDGFRa^H2B-GFP/+ double heterozygous alleles. (F-G) Longitudinal sections of Sca-1^{H2B-tdTomato/+};PDGFRa^H2B-GFP/+ mouse hearts at P60 (F) and P240 (G). Some Sca-1^H2B-tdTomato cells and PDGFRa^H2B-GFP cells showed co-localization (yellow, arrows in F2 and G2). (H) Flow cytometry analysis of 4-month-old Sca-1^{H2B-tdTomato/+};PDGFRa^H2B-GFP/+ mouse heart cells. (I) Diagram of the Sca-1^{H2B-tdTomato/+};Nkx2.5^H2B-GFP/+ double heterozygous alleles. (J, K) Longitudinal sections of Sca-1^{H2B-tdTomato/+};Nkx2.5^H2B-GFP/+ mouse hearts at P30 (M) and P120 (N). Sca-1^H2B-tdTomato cells (red, arrows in J2 and K2) and NKx2.5^H2B-GFP-positive cells (green, arrowheads in M2 and N2) were not co-localized. (L) Diagram of the Sca-1^{H2B-tdTomato/+};cTnT^H2B-GFP/+ double heterozygous alleles. (M, N) Longitudinal sections of Sca-1^{H2B-tdTomato/+};cTnT^H2B-GFP/+ mouse hearts at P30 (J) and P120 (K). No Sca-1^H2B-tdTomato cells (red, arrows in M2 and N2) were cTnT^H2B-GFP-positive (green, arrowheads in J2 and K2). LV, left ventricle; RV, right ventricle. n=3 for each stage. Scale bar, 100 μm.