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. Author manuscript; available in PMC: 2019 Dec 13.
Published in final edited form as: Cell. 2018 Dec 13;175(7):1917–1930.e13. doi: 10.1016/j.cell.2018.08.044

FIGURE 5. VP30 interacts with 549-571aa region of RBBP6.

FIGURE 5.

(A) Representative immunoblots after Co-IP between HA-VP30 and RBB6P peptide fused to GFP-flag. GFP-flag was used as a control.

(B) Interaction between RBBP6 peptide and NP binding mutants of VP30.

(C) Co-IP experiment demonstrating that NP interferes with VP30-RBBP6 peptide interaction. IP was performed with anti-flag beads after co-expression of VP30, RBBP6 peptide fused to GFP-flag and NP. IP: immunoprecipitation; IB: immunoblot; WCL: whole-cell lysate.

(D) Fluorescence polarization assay showing RBBP6 peptide (green) displaces FITC-NP peptide (red) from the eVP30CTD-FITC-NP peptide complex. Error bars represent standard deviation of three independent experiments.