KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-RBBP6 | Origene | Cat#TA309830; RRID:AB_2721115 |
| Mouse monoclonal anti-FLAG M2 antibody | Sigma Aldrich | Cat#F1804; RRID:AB_262044 |
| Rabbit polyclonal anti-flag antibody | Sigma Aldrich | Cat#F7425; RRID:AB_439687 |
| Mouse monoclonal anti-HA antibody | Sigma Aldrich | Cat#H3663; RRID:AB_262051 |
| Rabbit polyclonal anti-HA antibody | Sigma Aldrich | Cat#H6908; RRID:AB_260070 |
| Mouse monoclonal anti-β-tubulin antibody | Sigma Aldrich | Cat# T8328; RRID:AB_1844090 |
| Mouse monoclonal anti-myosinVa antibody | Santa Cruz | Cat#sc-365986; RRID:AB_10917581 |
| Mouse monoclonal anti-ANP32a antibody | Santa Cruz | Cat#sc-100767; RRID:AB_1124655 |
| Anti-KPNA5 antibody (1D2) | Santa Cruz | Cat#sc-517105; RRID:AB_2721174 |
| Rabbit anti-Ebola VP30 sera | This paper | N/A |
| Rabbit anti-Ebola NP sera | This paper | N/A |
| Anti-Ebola GP antibody clone 4F3 | IBT Bioservices | Cat#0201-020 |
| Goat IgG (H+L) anti-mouse Alexa Flour 546 conjugate | ThermoFisher scientific | Cat#A11030; RRID:AB_2534089 |
| Bacterial and Virus Strains | ||
| E. coli TOP10 | Invitrogen | Cat#C404003 |
| E. coli BL21(DE3) | Novagen | Cat#69450-3 |
| EBOV: Mayinga variant (GENBANK: NC_002549.1) | Texas Biomedical Research Institute | N/A |
| EBOV-EGFP: Mayinga variant (GENBANK: KF990213.1) | Heinz Feldmann (NIH, Hamilton, MT) | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Lipofectamine2000 | Invitrogen | Cat#11668500 |
| TransIT-X2 | Mirus | Cat#MIR6003 |
| Lipofectamine LTX | Thermo Fisher Scientific | Cat#15338500 |
| Lipofectamine RNAiMAX | Thermo Fisher Scientific | Cat#13778-150 |
| PolyJet | SignaGen Laboratories | Cat#SL100688 |
| Protease inhibitor cocktail | Sigma Aldrich (Roche) | Cat#11836170001 |
| RBBP6 peptide (SLPATPVFVPVPPPPLYPPPPHT) | Genscript | N/A |
| Perfecta SYBR Green Fastmix | VWR | Cat#101414-270 |
| Doxycycline | Clontech | Cat#631311 |
| PhosSTOP Phosphatase Inhibitor | Sigma-Aldrich | Cat#4906845001 |
| Nonidet P 40 Substitute | Fluka Analytical/Sigma-Aldrich | Cat#74385 |
| D-Desthiobiotin | IBA Lifesciences | Cat# 2-1000-001 |
| Sequencing Grade Modified Trypsin | Promega | Cat#V5113 |
| Critical Commercial Assays | ||
| QIAGEN Plasmid Plus Maxi Kit | Qiagen | Cat#12963 |
| RNeasy Mini Kit | Qiagen | Cat#74104 |
| QIAquick Gel Extraction Kit | Qiagen | Cat#28704 |
| QIAquick PCR Purification Kit | Qiagen | Cat#28104 |
| Dual-Glo® Luciferase Assay System | Promega | Cat#E2940 |
| QuikChange multi site-directed mutagenesis kit | Agilent Technologies | Cat#200514 |
| SuperScript® III First-Strand Synthesis System | Thermo Fisher Scientific | Cat#18080051 |
| Hampton Research crystal screen kits | Hampton research | Cat#HR2-110 |
| Neon transfection system 10 μl kit | Thermo Fisher Scientific | Cat#MPK1096 |
| Human Macrophage Nucleofector kit | Lonza | Cat#VPA-1008 |
| Pierce Silver Stain Kit | Thermo Scientific | Cat#24612 |
| Deposited Data | ||
| Crystal Structure of EBOV VP30 bound to RBBP6 peptide 553-567 | This paper | PDB: 6E5X |
| Experimental Models: Mammalian Cells | ||
| HEK293T | ATCC | CRL-3216 |
| HuH-7 | Frydman Lab | N/A |
| HeLa, female, authenticated by Bio-Synthesis, Inc | ATCC | CLL-2 |
| Vero | ATCC | CLL-81 |
| MDM | Texas Biomedical Research Institute | N/A |
| HUVEC | ATCC | Cat#PCS-100-013 |
| Oligonucleotides | ||
| Refer to Table S3. | n/a | n/a |
| Recombinant DNA | ||
| pCAGGS-RBBP6 isoform 1 | This paper | N/A |
| pCAGGS-RBBP6 isoform 3 | This paper | N/A |
| pCAGGS-NP | Hoenen et al.,2010; Edwards et al., 2015 | N/A |
| pCAGGS-VP30 | Hoenen et al.,2010; Edwards et al., 2015 | N/A |
| pCAGGS-VP35 | Hoenen et al.,2010; Edwards et al., 2015 | N/A |
| pCAGGS-L | Hoenen et al.,2010; Edwards et al., 2015 | N/A |
| pCAGGS-VP30 E197A | Xu et al., 2017 | N/A |
| pCAGGS-VP30 D202A | Xu et al., 2017 | N/A |
| pCAGGS-VP30 197/202/203AAA | Xu et al., 2017 | N/A |
| pCAGGS-VP30 Q229A | Xu et al., 2017 | N/A |
| pCAGGS-VP30 W230A | Xu et al., 2017 | N/A |
| pCAGGS-VP30 229/230AA | Xu et al., 2017 | N/A |
| pCAGGS-NP 610-612AAA | Xu et al., 2017 | N/A |
| pCAGGS-NP 603-606AAGG | Xu et al., 2017 | N/A |
| pCAGGS-VP30 R26A | This paper | N/A |
| pCAGGS-VP30 R28A | This paper | N/A |
| pCAGGS-VP30 R40A | This paper | N/A |
| pCAGGS-VP30 3RA | This paper | N/A |
| pCAGGS-VP30 C72S | This paper | N/A |
| pCAGGS-VP30 Reston virus | This paper | N/A |
| pCAGGS-VP30 Lloviu virus | This paper | N/A |
| pCAGGS-GFP-RBBP6 549-571aa | This paper | N/A |
| pMBP-VP30 truncations | Xu et al., 2017; this paper | N/A |
| pMBP-RBBP6 truncations | This paper | N/A |
| pLVX-TetOne-Puro-Nter2XStrep-ebovNP | This paper | N/A |
| pLVX-TetOne-Puro-Nter2XStrep-ebovVP35 | This paper | N/A |
| pLVX-TetOne-Puro-Nter2XStrep-ebovVP40 | This paper | N/A |
| pLVX-TetOne-Puro-Cter2XStrep-ebovGP | This paper | N/A |
| pLVX-TetOne-Puro-Cter2XStrep-ebovVP30 | This paper | N/A |
| pLVX-TetOne-Puro-Nter2XStrep-ebovVP24 | This paper | N/A |
| pLVX-TetOne-Puro-Nter2XStrep-ebovVP35-K319A-R322A | Leung et al., 2010; Prins et al., 2010 | N/A |
| pLVX-TetOne-Puro-Nter2XStrep-ebovVP35-Q74A | Kubota et al., 2009; Luthra et al., 2015 | N/A |
| pLVX-TetOne-Puro-Nter2XStrep-ebovVP24-F134A-M136A | Xu et al., 2014 | N/A |
| pLVX-TetOne-Puro-Nter2XStrep-ebovVP24-L201A-E203A-P204A-D205A-S207A | Xu et al., 2014 | N/A |
| pLVX-TetOne-Puro | This paper | N/A |
| pLVX-TetOne-Puro-Cter2XStrep-EGFP | This paper | N/A |
| Software and Algorithms | ||
| GraphPad Prism 7 | GraphPad Software | www.graphpad.com |
| CFX96 Real-Time PCR detection system | Bio-Rad | N/A |
| CellProfiler software | Broad Institute | http://cellprofiler.org/ |
| Nikon Ti-Eclipse microscope running high content analysis software | Nikon | https://www.nikoninstruments.com/Products/High-Content-Imaging-Systems/HCA-High-Content-Microscope-System |
| ORIGIN 7.0 software | OriginLab | https://www.originlab.com |
| CCP4 program suite | Collaborative Computational Project No.4 | http://www.ccp4.ac.uk/ |
| HKL3000 | HKL Research, Inc. | http://www.hkl-xray.com/hkl-3000 |
| PyMOL | Schrödinger | https://pymol.org/2/ |
| LigPlot+ | The European Bioinformatics Institute (EMBL-EBI) | https://www.ebi.ac.uk/thornton-srv/software/LigPlus/ |
| MiST algorithm | Jager et al., 2011 | n/a |
| Other | ||
| EASY-Column Capillary Columns (0.1mm × 20mm, 5um particles) | Thermo Scientific | Cat#SC001 |
| EASY-Column Capillary Columns (0.075mm × 100mm, 3um particles) | Thermo Scientific | Cat#SC200 |
| Strep-Tactin Sepharose 50% suspension | IBA Lifesciences | Cat#2-1201-010 |
| OMIX C18 pipette tips, 2 - 10 μL | Agilent | Cat#A5700310 |
| 4–20% Criterion Tris-HCl Protein Gel | Bio-Rad Laboratories | Cat#3450034 |
| 4–20% Criterion TGX Precast Gel | Bio-Rad Laboratories | Cat#5671095 |