Table 2. Stability results for 12 different saliva analytes obtained after measuring samples at different processing time and under different storage conditions.
|
Analyte (unit) |
T0 | Temperature | T1 | T2 | T3 | T4 | T5 | T6 | T7 | T8 | T9 | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 58,570 (47,055 – 101,200) | 71,340 (48,460 – 94,915) | 74,920 (44,770 – 92,445) | 79,850 (48,175 -100,690) | - | - | - | - | - | ||||
| RT | Variation (%) | 1.9 | 24.1 | 30.4 | 38.9 | - | - | - | - | - | ||
| P | > 0.999 | > 0.999 | > 0.999 | > 0.999 | - | - | - | - | - | |||
| 78,810 (48,060 – 102,305) | 68,670 (41,470 – 90,655) | 76,480 (39,935 – 95,885) | 79,970 (47,300 – 101,295) | 76,540 (47,395 – 99,160) | 81,000 (48,325 – 100,705) | - | - | - | ||||
| 4 ºC | Variation (%) | 37.1 | 19.5 | 33.1 | 39.2 | 33.2 | 40.9 | - | - | - | ||
| P | 0.494 | > 0.999 | > 0.999 | 0.769 | > 0.999 | > 0.999 | - | - | - | |||
| AMY (U/L) | 57,470 (39,615 – 101,310) |
- | - | - | - | - | 79,870 (47,165 – 100,730) | 68,840 (40,750 – 89,215) | 74,160 (42,280 – 97,095) | 77,610 (47,965 – 99,245) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 39.0 | 19.8 | 29.0 | 35.0 | ||
| P | - | - | - | - | - | > 0.999 | 0.054 | > 0.999 | > 0.999 | |||
| - | - | - | - | - | 79,300 (47,730 – 100,625) | 68,140 (39,715 – 87,200) | 71,250 (42,175 – 89,315) | 77,460 (49,580 – 104,120) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | 38.0 | 18.6 | 24.0 | 34.8 | ||
| P | - | - | - | - | - | > 0.999 | 0.156 | 0.248 | > 0.999 | |||
| 7.4 (3.7 -10.3) | 5.5 (0.1 – 7.7 | 5.3 (2.6 - 11.1) | 5.3 (3.6 – 11.9) | - | - | - | - | - | ||||
| RT | Variation (%) | 2.8 | 23.6 | 26.4 | 26.4 | - | - | - | - | - | ||
| P | > 0.999 | > 0.999 | > 0.999 | > 0.999 | - | - | - | - | - | |||
| 7.2 (3.6 -10.3) | 6.6 (3.2 – 8.8) | 11.2 (7.8 – 14.5) | 7.1 (4.0 – 10.7) | 11.4 (9.3 – 14.7) | 7.4 (6-6 – 10.3) | - | - | - | ||||
| 4 ºC | Variation (%) | 0.0 | 8.3 | 55.6 | 1.4 | 58.3 | 2.8 | - | - | - | ||
| P | > 0.999 | > 0.999 | 0.223 | > 0.999 | 0.097 | > 0.999 | - | - | - | |||
| CHE (µM/mL/min) | 7.2 (2.6 - 9.5) |
- | - | - | - | - | 4.9 (3.1 – 8.3) | 6.7 (4.2 – 8.0) | 5.9 (4.2 – 7.2) | 4.0 (0.6 – 4.8) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 31.9 | 6.9 | 18.1 | 44.4 | ||
|
Analyte (unit) |
T0 | Temperature | T1 | T2 | T3 | T4 | T5 | T6 | T7 | T8 | T9 | |
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | > 0.999 | |||
| - | - | - | - | - | 7.2 (6.1 – 11.3) | 8.1 (5.8 – 11.0) | 6.9 (4.9 – 10.1) | 4.5 (3.8 – 7.3) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | 0.0 | 12.5 | 4.2 | 37.5 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | > 0.999 | |||
| 10.6 (4.5 – 25.5) | 11.6 (4.3 – 15.9) | 4.4 (3.6 – 8.2) | 4.1 (3.6 -4.7) | - | - | - | - | - | ||||
| RT | Variation (%) | 20.9 | 13.4 | 67.2 | 69.4 | - | - | - | - | - | ||
| P | > 0.999 | > 0.999 | < 0.001 | < 0.001 | - | - | - | - | - | |||
| 14.3 (5.1 -23.3) | 15.7 (3.7 - 22.1) | 6.3 (2.4 – 16) | 2.1 (0.4 - 3.3) | 1.7 (1.4 - 4.1) | 0.6 (0.5 - 3.9) | - | - | - | ||||
| 4 ºC | Variation (%) | 6.7 | 17.2 | 53.0 | 84.3 | 87.3 | 95.5 | - | - | - | ||
| P | > 0.999 | > 0.999 | < 0.001 | < 0.001 | <0.001 | < 0.001 | - | - | - | |||
| Lip (U/L) | 13.4 (7.3 - 33.2) |
- | - | - | - | - | 3.8 (0.9 – 8.0) | 2.0 (1.4 – 14.0) | 2.0 (1.2 - 3.5) | 3.5 (2.4 - 10.6) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 71.6 | 85.1 | 85.1 | 73.9 | ||
| P | - | - | - | - | - | 0.018 | 0.017 | < 0.001 | 0.031 | |||
| - | - | - | - | - | 12.5 (3.5 - 32.4) | 9.9 (2.7 - 36.4) | 11.6 (4.1 - 34.7) | 12.9 (4.5 - 54.4) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | 6.7 | 26.1 | 13.4 | 3.7 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | > 0.999 | |||
| 20.1 (14.4 -48.8) | 15.7 (12.4 - 39.1) | 27.7 (23.8 - 32.4) | 18.1 (16.4 - 25.4) | - | - | - | - | - | ||||
| RT | Variation (%) | 9.9 | 29.3 | 24.5 | 18.6 | - | - | - | - | - | ||
| P | > 0.999 | 0.002 | > 0.999 | 0.035 | - | - | - | - | - | |||
| 19.7 (15.7 - 51.3) | 16.4 (13.3 - 42.4) | 28.8 (25.4 - 57.4) | 14.8 (12.2 - 27.2) | 26.5 (20.4 -42.6) | 23.2 (18.4 - 34.2) | - | - | - | ||||
| 4 ºC | Variation (%) | 11.5 | 26.5 | 29.2 | 33.7 | 18.9 | 4.3 | - | - | - | ||
| P | > 0.999 | 0.041 | > 0.999 | < 0.001 | > 0.999 | > 0.999 | - | - | - | |||
|
Analyte (unit) |
T0 | Temperature | T1 | T2 | T3 | T4 | T5 | T6 | T7 | T8 | T9 | |
| TEA (U/L) | 22.3 (15.6 - 59.4) |
- | - | - | - | - | 19.9 (15.6 - 49.7) | 24.4 (17 - 56.9) | 16.8 (9.9 -35.4) | 21.5 (13.9 -43.4) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 10.3 | 9.7 | 24.5 | 3.4 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | < 0.001 | 0.315 | |||
| - | - | - | - | - | 20.5 (15.4 - 50.9) | 24.3 (17.8 - 56.7) | 13.6 (9.0 -34.2) | 21.0 (15.6 -53) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | 8.1 | 9.0 | 38.9 | 5.6 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | < 0.001 | > 0.999 | |||
| 9.1 (3.6 - 21.6) | 10.0 (3.8 - 20.4) | 5.5 (1.5 - 9.8) | 6.0 (2.4 - 11.2) | - | - | - | - | - | ||||
| RT | Variation (%) | 45.5 | 40.1 | 67.2 | 63.9 | - | - | - | - | - | ||
| P | > 0.999 | 0.248 | 0.002 | 0.003 | - | - | - | - | - | |||
| 11.7 (5.8 - 21.4) | 11.5 (6.6 - 16.5) | 10.9 (4.7 - 18.0) | 5.7 (2.7 - 10.1) | 4.4 (0.9 - 7.8) | 2.0 ((- 1.0) -7.9) | - | - | - | ||||
| 4 ºC | Variation (%) | 29.8 | 30.7 | 34.6 | 66.0 | 70.8 | 88.0 | - | - | - | ||
| P | > 0.999 | > 0.999 | > 0.999 | 0.006 | <0.001 | < 0.001 | - | - | - | |||
| CK (U/L) | 16.6 (7.00 - 33.2) |
- | - | - | - | - | 7.0 (3.4 - 10.8) | 5.9 (4.7 -10.2) | 7.2 (5.7 - 11.5) | 6.9 (4.0 - 8.5) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 57.8 | 64.8 | 56.6 | 58.4 | ||
| P | - | - | - | - | - | 0.003 | 0.043 | 0.045 | 0.020 | |||
| - | - | - | - | - | 12.9 (6.7 - 22.4) | 13.6 (8.7 - 20.7) | 15.9 (8.1 - 20.5) | 13.6 (10.1 - 20.1) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | 22.6 | 18.1 | 4.2 | 18.4 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | > 0.999 | |||
| 11.2 (9.0 - 25.5) | 11.5 (10.2 - 24.0) | 15.3 (12.4 - 24.7) | 22.2 (15.3 - 32.5) | - | - | - | - | - | ||||
| RT | Variation (%) | 7.1 | 4.6 | 27.0 | 83.8 | - | - | - | - | - | ||
|
Analyte (unit) |
T0 | Temperature | T1 | T2 | T3 | T4 | T5 | T6 | T7 | T8 | T9 | |
| P | > 0.999 | > 0.999 | > 0.999 | > 0.999 | - | - | - | - | - | |||
| 11.7 (9.5 - 25.2) | 12.7 (10.5 - 24.4) | 12.8 (10.3 - 23.6) | 10.3 (8.3 - 24.1) | 10.7 (8.4 - 29.3) | 10.3 (8.2 - 24.7) | - | - | - | ||||
| 4 ºC | Variation (%) | 3.3 | 5.0 | 6.2 | 14.5 | 11.2 | 14.9 | - | - | - | ||
| P | > 0.999 | > 0.999 | > 0.999 | 0.760 | 0.508 | 0.222 | - | - | - | |||
| AST (U/L) | 12.1 (10.1 - 26.7) |
- | - | - | - | - | 9.7 (7.7 - 17.2) | 9.1 (5.9 - 11.3) | 7.9 (6.1 - 11.5) | 5.2 (2.8 - 6.0) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 19.5 | 24.9 | 34.4 | 57.3 | ||
| P | - | - | - | - | - | 0.028 | < 0.001 | < 0.001 | < 0.001 | |||
| - | - | - | - | - | 12.8 (11.0 - 25.1) | 13.1 (11.1 - 26.1) | 13.5 (11.4 - 26.1) | 13.9 (9.7 - 25.0) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | 6.2 | 8.3 | 12.0 | 15.4 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | 0.666 | |||
| 338 (214 -409) | 339 (212 – 399) | 276 (189 – 344) | 204 (139 – 295) | - | - | - | - | - | ||||
| RT | Variation (%) | 0.3 | 0.6 | 18.1 | 39.3 | - | - | - | - | - | ||
| P | > 0.999 | > 0.999 | 0.365 | 0.006 | - | - | - | - | - | |||
| 333 (224- 402) | 336 (193 – 404) | 320 (211 – 383) | 220 (172 – 338) | 196 (156 – 302) | 131 (77 – 261) | - | - | - | ||||
| 4 ºC | Variation (%) | 1.0 | 0.1 | 5.0 | 34.8 | 41.7 | 61.1 | - | - | - | ||
| P | > 0.999 | > 0.999 | > 0.999 | 0.222 | 0.006 | < 0.001 | - | - | - | |||
| LD (U/L) | 337 (221-403) |
- | - | - | - | - | 69 (27 – 168) | 54 (18 – 97) | 27 (5 – 59) | 31 (15 – 59) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 79.5 | 84.0 | 92.1 | 90.9 | ||
| P | - | - | - | - | - | < 0.001 | < 0.001 | < 0.001 | < 0.001 | |||
| - | - | - | - | - | 323 (214 – 391) | 321 (223 – 399) | 335 (234 – 414) | 370 (279 – 445) | ||||
|
Analyte (unit) |
T0 | Temperature | T1 | T2 | T3 | T4 | T5 | T6 | T7 | T8 | T9 | |
| - 80 ºC | Variation (%) | - | - | - | - | - | 4.0 | 1.8 | 0.6 | 10.0 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | > 0.999 | |||
| 0.5 (0.4 - 1.3) | 0.5 (0.4 - 1.3) | 0.3 (0.1 - 1.2) | 0.01((- 0.003) -0.04) | - | - | - | - | - | ||||
| RT | Variation (%) | 1.0 | 1.0 | 58.2 | 98.1 | - | - | - | - | - | ||
| P | > 0.999 | > 0.999 | 0.634 | 0.003 | - | - | - | - | - | |||
| 0.5 (0.4 - 1.2) | 0.5 (0.4 - 1.2) | 0.4 (0.3 -1.3) | 0.4 (0.3 - 1.4) | 0.5 (0.4 -1.5) | 0.5 (0.1 - 0.7) | - | - | - | ||||
| 4 ºC | Variation (%) | 1.9 | 1.0 | 15.5 | 17.5 | 8.7 | 9.7 | - | - | - | ||
| P | > 0.999 | > 0.999 | > 0.999 | > 0.999 | >0.999 | > 0.999 | - | - | - | |||
|
Lact (mmol/L) |
0.5 (0.4 - 1.2) |
- | - | - | - | - | 0.5 (0.4 - 1.2) | 0.5 (0.4 - 1.1) | 0.5 (0.4 - 1.2) | 0.5 (0.4 - 1.2) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 1.0 | 10.7 | 5.8 | 3.9 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | > 0.999 | |||
| - | - | - | - | - | 0.5 (0.4 - 1.2) | 0.5 (0.4 - 1.2) | 0.5 (0.4 - 1.2) | 0.5 (0.4 -1.2) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | 1.0 | 6.8 | 1.9 | 3.9 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | > 0.999 | |||
| 1.2 (0.8 - 1.7) | 1.3 (1.0 - 1.9) | 2.0 (1.2 -2.2) | 1.1 (0.9 - 1.8) | - | - | - | - | - | ||||
| RT | Variation (%) | 18.2 | 35.4 | 101 | 6.1 | - | - | - | - | - | ||
| P | > 0.999 | 0.059 | 0.007 | > 0.999 | - | - | - | - | - | |||
| 1.1 (0.9 - 1.6) | 1.2 (0.8 - 1.8) | 1.0 (0.8 - 1.9) | 0.9 (0.6 - 1.7) | 0.8 (0.6 -1.2) | 0.6 (0.5 - 1.2) | - | - | - | ||||
| 4 ºC | Variation (%) | 12.1 | 21.2 | 4.0 | 6.1 | 20.2 | 37.4 | - | - | - | ||
| P | > 0.999 | 0.734 | > 0.999 | > 0.999 | >0.999 | > 0.999 | - | - | - | |||
|
Analyte (unit) |
T0 | Temperature | T1 | T2 | T3 | T4 | T5 | T6 | T7 | T8 | T9 | |
|
ADA (U/L) |
1.0 (0.7 - 1.6) |
- | - | - | - | - | 1.0 (0.5 - 1.7) | 0.8 (0.3 - 1.3) | 0.5 (0.2 - 1.1) | 0.6 (0.2 - 0.8) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 2.0 | 15.2 | 45.5 | 41.4 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | 0.189 | 0.064 | |||
| - | - | - | - | - | 1.2 (0.8 - 1.8) | 1.2 (0.8 - 1.8) | 1.0 (0.7 - 1.5) | 1.2 (1.0 - 2.0) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | 17.2 | 16.2 | 2.0 | 18.2 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | 0.291 | |||
| 0.2 (0.2 - 0.3) | 0.2 (0.2 - 0.3) | 0.1 (0.1 - 0.2) | 0.2 (0.1 - 0.2) | - | - | - | - | - | ||||
| RT | Variation (%) | 31.6 | 26.2 | 13.9 | 9.1 | - | - | - | - | - | ||
| P | 0.701 | 0.529 | 0.582 | 0.582 | - | - | - | - | - | |||
| 0.2 (0.2 - 0.3) | 0.2 (0.2 - 0.3) | 0.2 (0.1 - 0.2) | 0.2 (0.1 - 0.2) | 0.2 (0.2 -0.3) | 0.2 (0.1 - 0.2) | - | - | - | ||||
| 4 ºC | Variation (%) | 29.5 | 24.1 | 4.6 | 7.4 | 17.1 | 10.5 | - | - | - | ||
| P | > 0.999 | > 0.999 | > 0.999 | > 0.999 | 0.064 | > 0.999 | - | - | - | |||
|
TEAC (mM) |
0.2 (0.1 – 0.2) |
- | - | - | - | - | - | 0.2 (0.1 - 0.2) | 0.2 (0.1 - 0.2) | 0.2 (0.1 - 0.3) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | - | 6.0 | 6.8 | 33.8 | ||
| P | - | - | - | - | - | - | > 0.999 | > 0.999 | 0.457 | |||
| - | - | - | - | - | - | 0.2 (0.1 - 0.2) | 0.2 (0.1 - 0.3) | 0.2 (0.2 - 0.3) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | - | 8.3 | 11.0 | 32.0 | ||
| P | - | - | - | - | - | - | > 0.999 | 0.291 | 0.841 | |||
| 0.4 (0.3 - 0.5) | 0.4 (0.3 - 0.5) | 0.4 (0.3 - 0.5) | 0.3 (0.2 - 0.3) | - | - | - | - | - | ||||
| RT | Variation (%) | 3.3 | 9.1 | 9.5 | 13.3 | - | - | - | - | - | ||
|
Analyte (unit) |
T0 | Temperature | T1 | T2 | T3 | T4 | T5 | T6 | T7 | T8 | T9 | |
| P | > 0.999 | 0.669 | > 0.999 | < 0.001 | - | - | - | - | - | |||
| 0.4 (0.2 - 0.5) | 0.3 (0.2 - 0.5) | 0.4 (0.3 - 0.5) | 0.3 (0.3 - 0.4) | 0.3 (0.2 -0.4) | 0.4 (0.3 - 0.4) | - | - | - | ||||
| 4 ºC | Variation (%) | 3.0 | 1.3 | 2.0 | 4.5 | 9.4 | 4.3 | - | - | - | ||
| P | > 0.999 | > 0.999 | > 0.999 | 0.555 | 0.018 | 0.047 | - | - | - | |||
|
FRAS (mM) |
0.3 (0.3 - 0.5) |
- | - | - | - | - | 0.3 (0.3 - 0.5) | 0.4 (0.3 - 0.5) | 0.3 (0.3 - 0.5) | 0.3 (0.3 - 0.5) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 0.1 | 10.4 | 4.8 | 0.3 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | > 0.999 | |||
| - | - | - | - | - | 0.3 (0.2 - 0.5) | 0.3 (0.2 - 0.5) | 0.3 (0.2 - 0.5) | 0.3 (0.2 - 0.5) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | 0.1 | 0.6 | 1.3 | 2.1 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | 0.394 | |||
| 0.2 (0.2 - 0.2) | 0.2 (0.1 - 0.2) | 0.2 (0.1 - 0.2) | 0.2 (0.1 - 0.2) | - | - | - | - | - | ||||
| RT | Variation (%) | 1.1 | 1.5 | 0.1 | 27.2 | - | - | - | - | - | ||
| P | > 0.999 | > 0.999 | 0.010 | < 0.001 | - | - | - | - | - | |||
| 0.2 (0.2 - 0.2) | 0.2 (0.1 - 0.2) | 0.2 (0.1 - 0.2) | 0.2 (0.1 - 0.2) | 0.2 (0.1 -0.2) | 0.2 (0.1 - 0.2) | - | - | - | ||||
| 4 ºC | Variation (%) | 0.8 | 0.1 | 5.9 | 12.7 | 14.3 | 10.1 | - | - | - | ||
| P | > 0.999 | 0.794 | 0.002 | < 0.001 | <0.001 | < 0.001 | - | - | - | |||
| CUPRAC (mM) | 0.2 (0.2 - 0.3) |
- | - | - | - | - | 0.2 (0.2 - 0.2) | 0.2 (0.2 - 0.2) | 0.2 (0.2 - 0.2) | 0.2 (0.2 - 0.3) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 1.2 | 0.3 | 3.3 | 2.1 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | 0.162 | > 0.999 | |||
| - | - | - | - | - | 0.2 (0.2 - 0.2) | 0.2 (0.2 - 0.3) | 0.2 (0.2 - 0.2) | 0.2 (0.2 - 0.3) | ||||
|
Analyte (unit) |
T0 | Temperature | T1 | T2 | T3 | T4 | T5 | T6 | T7 | T8 | T9 | |
| - 80 ºC | Variation (%) | - | - | - | - | - | 0.1 | 3.7 | 0.2 | 2.4 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | 0.131 | > 0.999 | |||
| 13.1 (9.5 - 16.7) | 13.7 (9.5 - 16.7) | 11.9 (7.7 - 16.1) | 9.5 (5.9 - 12.5) | - | - | - | - | - | ||||
| RT | Variation (%) | 2.6 | 1.3 | 12.2 | 28.6 | - | - | - | - | - | ||
| P | > 0.999 | > 0.999 | 0.084 | < 0.001 | - | - | - | - | - | |||
| 13.7 (9.5 - 17.3) | 13.1 (9.5 - 17.3) | 13.1 (8.9 - 16.7) | 12.5 (8.3 - 16.1) | 11.9 7.7 - 14.3) | 10.7 (7.1 - 13.7) | - | - | - | ||||
| 4 ºC | Variation (%) | 1.7 | 1.5 | 4.6 | 10.2 | 12.1 | 20.6 | - | - | - | ||
| P | > 0.999 | > 0.999 | > 0.999 | 0.383 | 0.014 | 0.002 | - | - | - | |||
| Uric acid (µmol/L) | 13.7 (9.5 - 16.1) |
- | - | - | - | - | 13.7 (9.5 - 18.4) | 13.7 (9.5 - 16.7) | 14.3 (9.5 - 17.3) | 14.3 (10.1 - 17.8) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 1.5 | 2.4 | 2.8 | 5.6 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | 0.365 | |||
| - | - | - | - | - | 13.7 (9.5 - 16.7) | 13.1 (9.5 - 16.7) | 13.7 (9.5 - 17.3) | 14.3 (9.5 - 16.7) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | 0.2 | 2.8 | 0.0 | 2.8 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | 0.666 | |||
| 0.2 (0.2 - 0.3) | 0.2 (0.2 - 0.3) | 0.2 (0.2 - 0.4) | 0.4 (0.2 - 0.5) | - | - | - | - | - | ||||
| RT | Variation (%) | 2.2 | 13.3 | 12.5 | 50.0 | - | - | - | - | - | ||
| P | > 0.999 | 0.960 | > 0.999 | > 0.999 | - | - | - | - | - | |||
| 0.2 (0.2 - 0.3) | 0.2 (0.2 - 0.3) | 0.3 (0.2 - 0.3) | 0.2 (0.2 - 0.3) | 0.3 (0.2 - 0.4) | 0.2 (0.2 - 0.4) | - | - | - | ||||
| 4 ºC | Variation (%) | 8.9 | 1.2 | 2.0 | 6.5 | 33.5 | 0.4 | - | - | - | ||
| P | > 0.999 | > 0.999 | > 0.999 | > 0.999 | >0.999 | > 0.999 | - | - | - | |||
|
Analyte (unit) |
T0 | Temperature | T1 | T2 | T3 | T4 | T5 | T6 | T7 | T8 | T9 | |
| CAT (units/mL) | 0.2 (0.2 – 0.3) |
- | - | - | - | - | 0.2 (0.2 - 0.3) | 0.3 (0.2 - 0.4) | 0.3 (0.2 - 0.4) | 0.3 (0.2 - 0.4) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 8.1 | 14.1 | 19.4 | 26.2 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | > 0.999 | |||
| - | - | - | - | - | 0.3 (0.2 - 0.3) | 0.3 (0.2 - 0.4) | 0.2 (0.2 - 0.4) | 0.2 (0.2 - 0.4) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | 4.0 | 11.3 | 2.4 | 4.8 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | > 0.999 | |||
| 103 (80 – 169) | 106 (83 – 168) | 121 (79 – 147) | 59 (41 – 69) | - | - | - | - | - | ||||
| RT | Variation (%) | 6.7 | 3.7 | 9.8 | 46.9 | - | - | - | - | - | ||
| P | > 0.999 | > 0.999 | > 0.999 | 0.001 | - | - | - | - | - | |||
| 116 (81 – 169) | 119 (89 – 165) | 115 (67 – 142) | 102 (60 – 126) | 109 (56 – 173) | 95 (69 – 123) | - | - | - | ||||
| 4 ºC | Variation (%) | 4.6 | 8.1 | 4. | 8.1 | 1.2 | 14.2 | - | - | - | ||
| P | > 0.999 | > 0.999 | 0.914 | 0.331 | >0.999 | > 0.999 | - | - | - | |||
|
AOPP (μM) |
111 (76 -180) |
- | - | - | - | - | 109 (97 – 184) | 115 (100 – 186) | 102 (81 – 155) | 96 (82 – 146) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 1.9 | 4.1 | 7.3 | 13.3 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | > 0.999 | |||
| - | - | - | - | - | 114 (90 – 185) | 116 (98 – 182) | 110 (84 – 171) | 124 (83 – 207) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | 2.9 | 4.7 | 0.5 | 11.8 | ||
| P | - | - | - | - | - | > 0.999 | > 0.999 | > 0.999 | > 0.999 | |||
| 5.4 (2.1 - 6.6) | 6.8 (3.2 -10.6) | 5.6 (4.3 -10.8) | 4.4 (2.2 - 7.2) | - | - | - | - | - | ||||
| RT | Variation (%) | 120.4 | 178.8 | 127.3 | 80.8 | - | - | - | - | - | ||
|
Analyte (unit) |
T0 | Temperature | T1 | T2 | T3 | T4 | T5 | T6 | T7 | T8 | T9 | |
| P | > 0.999 | 0.011 | 0.001 | > 0.999 | - | - | - | - | - | |||
| 5.3 (2.4 - 6.4) | 7.3 (3.1 -10.2) | 8.6 (5.7 -12.2) | 6.1 (3.2 - 7.6) | 4.2 (3.5 -7.2) | 4.5 (2.5 - 8.0) | - | - | - | ||||
| 4 ºC | Variation (%) | 117.1 | 198 | 252.2 | 146.9 | 72.2 | 84.5 | - | - | - | ||
| P | > 0.999 | 0.012 | < 0.001 | 0.960 | >0.999 | > 0.999 | - | - | - | |||
|
H2O2 (μM) |
2.5 (1.7 - 8.9) |
- | - | - | - | - | 6.8 (2.1 - 9.4) | 7.5 (3.1 - 9.0) | 9.2 (4.1 -11.8) | 7.7 (3.8 -10.8) | ||
| - 20 ºC | Variation (%) | - | - | - | - | - | 176.3 | 206.9 | 274.7 | 212.2 | ||
| P | - | - | - | - | - | > 0.999 | 0.248 | < 0.001 | 0.002 | |||
| - | - | - | - | - | 6.2 (2.5 -10.8) | 6.6 (2.8 - 9.0) | 7.2 (3.3 -12.4) | 9.6 (3.0 -12.2) | ||||
| - 80 ºC | Variation (%) | - | - | - | - | - | 153.5 | 169.8 | 193.9 | 291.0 | ||
| P | - | - | - | - | - | 0.804 | > 0.999 | < 0.001 | 0.002 | |||
| Quantitative data is presented as median and interquartile range. T0 - baseline. T1 - 3 hours. T2 - 6 hours. T3 - 24 hours.T4 - 72 hours T5 - 7 days. T6 - 14 days. T7 – 1 month . T8 – 3 months . T9 – 6months. RT - room temperature. AMY - α-amylase. CHE - cholinesterase. Lip - lipase. TEA - total esterase. CK - creatine kinase. AST - aspartate aminotransferase. LD - lactate dehydrogenase. Lact - lactate. ADA - adenosine deaminase. TEAC - Trolox equivalent antioxidant capacity. FRAS - ferric reducing ability. CUPRAC - cupric reducing antioxidant capacity. CAT - catalase. AOPP - advanced oxidation protein products. H2O2 - hydrogen peroxide. P < 0.05 represents statistical significant difference. | ||||||||||||