. 2019 Feb 15;29(1):010707. doi: 10.11613/BM.2019.010707

Table 3. Evaluation of extraction yield depending on incubation time and initial sample dilution.

Extraction yield with different sample incubation time in extraction buffer, N = 20
	FC concentration, mg/kg	P*	Mean bias, %
EliA™ Calprotectin, 10 min	128.5 (39.8 - 334.0)	0.019	16.5
EliA™ Calprotectin, 1 hour	155.0 (44.0 - 378.0)	0.019	16.5
Bühlmann fCAL^® Turbo, 10 min	112.0 (54.0 - 281.5)	< 0.001	6.5
Bühlmann fCAL^® Turbo, 1 hour	120.5 (56.0 - 319.0)	< 0.001	6.5
Extraction yield with different initial FC sample dilution, Bühlmann fCAL Turbo, N = 13
	FC concentration, mg/kg	P^†	Multiple comparison *(Conover post-hoc)*
Weighing 1/50 (1)	361.0 (63.5 - 676.0)	< 0.001	(1)/ (2)/ (3) (4)
Weighing 1/100 (2)	378.0 (81.5 - 884.5)
Weighing 1/500 (3)	384.0 (81.8 - 1009.3)
Calex^® Cap “N” (4)	403.0 (94.0 - 913.0)
FC-faecal calprotectin concentration is presented as median (interquartile range). Wilcoxon test. Mean bias - mean of calculated biases (Bias = [(FC_1hour – FC_10min) / FC_10min ] x 100). ^†Friedman ANOVA with Conover post hoc*. P < 0.05 was considered statistically significant. The allowable bias was set at 22.6%. (1)/ (2)/ (3) (4) - median FC value of 1/50 group is significantly different from median of 1/100 group and this two medians are significantly different from medians of 1/500 and Calex^® Cap “N” group.