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High glucose alters microglia polarization with time. (A) The microglia cell line HMC3 was cultured in a normal physiological glucose level (80 mg/dl G) and high glucose level (350 mg/dl G), respectively, for 288 hours. Some cells were harvested every 48 hours. (B–G) RT-qPCR for iNOS (B), TNF-α (c), IL-6 (D), IL-12 (E), IL-10 (F), Arg-1 (G) and CD206 (H) mRNA levels in HMC3 cells at different time points. N=5. *p<0.05.
