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. Author manuscript; available in PMC: 2019 Feb 22.
Published in final edited form as: Leukemia. 2018 Aug 22;33(2):426–438. doi: 10.1038/s41375-018-0242-6

Figure 2. APRIL signals via TACI to significantly increase viability and inhibit apoptosis in Treg vs paired Tcon.

Figure 2.

Purified Treg and Tcon cells from the same patient were incubated with recombinant human APRIL in media containing low dose IL-2 (5 ng/ml) with or without neutralizing anti-APRIL mAb (A1, clone 01A) followed by luminescence CellTiter-Glo (CTG) (left) and [3H] thymidine incorporation (right) assays (a), as well as CTG-based caspase activity assay (b). Similar results were obtained from additional 3 samples. (c) Purified Tregs and paired Tcons (n=2) were incubated with APRIL for 6h and 1d in the presence or absence of blocking anti-APRIL mAbs (A1, A2). Expression levels of indicated genes were then determined using qRT-PCR normalized by internal controls GAPDH. cnt, control media * p < 0.02, ** p < 0.005, *** p < 0.001, **** p < 0.0001.

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