Figure 4. APRIL potentiates Treg-inhibited proliferation of autologous Tcon cells.
a, Purified Tcons were stained with 5μM CFSE and then stimulated with CD3/CD28 beads (beads) in the presence or absence of autologous Tregs at indicated ratios of Treg/Tcon, with or without APRIL (200 ng/ml). b, Beads-stimulated Tcons were cocultured with autologous Tregs for 7d at lower ratios of Treg/Tcon in serial dilutions of APRIL (μg/ml). c, Tcons were cocultured with Tregs at a low Treg/Tcon ratio with APRIL (μg/ml) in the presence or absence of neutralizing anti-APRIL mAb (μg/ml) for 7d. C1, chimeric homolog of A1 (01A). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.