Skip to main content
. Author manuscript; available in PMC: 2019 Feb 22.
Published in final edited form as: Leukemia. 2018 Aug 22;33(2):426–438. doi: 10.1038/s41375-018-0242-6

Figure 6. Osteoclasts induce generation of iTreg dependent on APRIL secretion.

Figure 6.

(a) Osteoclasts (OC) were differentiated from CD14+ cells following 3-week stimulation with M-CSF and RANKL and then co-cultured with autologous T cells for 7d in the presence or absence of anti-APRIL mAbs (A1, 10 μg/ml). Generation of iTreg was determined by gating CD25+Foxp3+ in CD4+ T cells. (b), CD3 T cells were cultured with supernatants (S) from 3-week OC cultures from the same donors for 7d under indicated conditions. Percentages of CD25+Foxp3+ iTreg in CD4+ T were determined. (c) CD3 T cells, pre-stained with CFSE, were co-cultured with OCs from the same donor under indicated conditions for 7d followed by flow cytometric analysis to determine fractions of proliferative Tcons. When noted, antagonistic anti-APRIL mAbs A1 or A2 (50 μg/ml) or anti(α)-PD-L1 or -PD1 mAbs (10 μg/ml) were added. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Shown here are data from 1 representative experiment with triplicates for each condition. Similar results were obtained using 2 additional samples.

HHS Vulnerability Disclosure