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. 2018 Oct 25;221(4):2080–2095. doi: 10.1111/nph.15497

Figure 4.

Figure 4

Signaling components playing similar roles in nlp20‐ and flg22‐induced immunity. (a) Leaf disks of Arabidopsis wild‐type (WT) plants or the mutant lines bak1‐4, bak1‐5, bak1‐4/BAK1, bak1‐4/BAK1_Y403F, bak1‐4/BAK1_S602/3/4_AAA and bak1‐4/BAK1_S612A were treated with 0.5 μM flg22 or nlp20, respectively, and reactive oxygen species (ROS) accumulation was determined. (b–d) ROS production was compared in WT Arabidopsis plants vs the mutant lines (b) bir2‐1, (c) pp2a‐a1, pp2a‐c4, and cpk28, or (d) xlg2‐1, agg1 agg2 and agb1‐2, each treated with either 0.5 μM flg22 or nlp20. All presented data (relative fluorescence units, RLU) show peak values minus background values as dots (n ≥ 6) and the means as lines. Asterisks indicate significant differences compared with the wild‐type as determined by Student's t‐test: *, P < 0.05; **, P < 0.01; ***, P < 0.001. The experiments were performed three times with similar results.