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Observed F4 outcomes of germline Cas9 strategies.
| Cre | Cas9 | F3 parent (F)emale or (M)ale |
No cut or functional repair |
NHEJ disruption |
HDR conversion |
%HDR conversion observed |
|
|---|---|---|---|---|---|---|---|
| Vasa | Rosa26 | F | 1 | - | 15 | - | - |
| 2 | 1 | 2 | - | - | |||
| 3 | - | 3 | 1 | 25% | |||
| 4 | 4 | 5 | 3 | 25% | |||
| 5 | 6 | 6 | 1 | 8% | |||
| M | 1 | - | 25 | - | - | ||
| 2 | - | 17 | - | - | |||
| H11 | F | 1 | 4 | 1 | 13 | 72% | |
| 2 | 10 | 7 | 4 | 19% | |||
| 3 | 4 | - | 5 | 56% | |||
| 4 | 8 | 4 | 4 | 25% | |||
| 5 | 8 | 3 | 10 | 48% | |||
| M | 1 | - | 15 | - | - | ||
| 2 | - | 5 | - | - | |||
| 3 | - | 2 | - | - | |||
| 4 | - | 3 | - | - | |||
| Stra8 | Rosa26 | F | 1 | - | 19 | - | - |
| 2 | - | 3 | - | - | |||
| H11 | M | 1 | 3 | 21 | - | - | |
