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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: New Phytol. 2018 Nov 5;221(4):2160–2175. doi: 10.1111/nph.15523

Figure 5.

Figure 5.

The bik1pbl1 double mutant does not exhibit a defect in reactive oxygen species (ROS) accumulation during effector-triggered immunity (ETI). (a) BOTRYTIS-INDUCED KINASE-1 (BIK1) protein accumulates after infection with Pseudomonas syringae pv tomato (Pto) DC3000 (avrRpm1). pBIK1:BIK1-HA plants were inoculated with 5 mM MgCl2, Pto DC3000 EV or Pto DC3000 (avrRpm1) (2.5 × 107 cfu (colony-forming units) ml−1) and BIK1-HA protein amount was determined by immunoblot analyses using anti HA antibody. (b) H2O2 accumulation in Col-0 and bik1pbl1 mutant after inoculation with 10 mM MgCl2, Pto DC3000 EV or Pto DC3000 (avrRpm1) (2.5 × 107 cfu ml−1). H2O2 accumulation was detected by 3,3’-diaminobenzidine (DAB). The experiments were performed three times with similar results.