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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: New Phytol. 2018 Nov 5;221(4):2160–2175. doi: 10.1111/nph.15523

Figure 7.

Figure 7.

RESPIRATORY BURST OXIDASE HOMOLOGUE D (RBOHD) phosphorylation sites S343 and S347 are required for effector-triggered immunity (ETI). Bacterial growth of Pseudomonas syringae pv tomato (Pto) DC3000 (avrRpm1) (a) and Pto DC3000 (avrRpt2) (b) in rbohD and rbohD expressing 3xFLAG-RBOHD WT or the S343A/S347A variant. Bacteria were syringe infiltrated in three leaves per one plant at a concentration of 1 × 105 cfu (colony-forming units) ml−1. Three days post infiltration, leaves were harvested to determine bacterial growth. Data are means ±SD of 8 replicates. Solid horizontal lines within the boxes show median. Different letters indicate significantly different values at p ≤ 0.01 (one-way ANOVA, Tukey post hoc test). These experiments were repeated three times with similar results.