Figure 8.

The double mutant rbohDrbohF shows weakened resistance to virulent Plectosphaerella cucumerina (PcBMM isolate). (a) Symptoms of 18-d-old plants after spray inoculation with PcBMM (4 × 10⁶ spores ml⁻¹). (b) Quantification of PcBMM biomass. Fungal DNA was quantified by qPCR at 6 d post-inoculation (dpi) using specific primers for PcBMM β-TUBULIN and normalized to Arabidopsis thaliana UBIQUITIN 10 gene. Bars represent averages (±SE) of fungal DNA levels relative to Col-0 plants from two replicates. Statistical analysis was performed by ANOVA, corrected with Bonferroni post hoc test. Different letters indicate significant differences (p ≤ 0.05). The experiment was repeated twice with similar results.