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. Author manuscript; available in PMC: 2019 Jun 30.
Published in final edited form as: Nature. 2018 Dec 31;566(7742):136–139. doi: 10.1038/s41586-018-0856-x

Fig. 1 |. Overall structure of the Sec complex.

Fig. 1 |

a, The Sec complex was purified by utilizing a FLAG-tag on Sec63, followed by gel filtration (left panel). The indicated fractions were combined and subjected to SDS-PAGE and Coomassie blue staining (lane 1). Endoglycosidase H treatment (lane 2) cleaves the glycan from Sec71 (Sec71*). The experiment was independently repeated five times. b, Side view of the density map with regions of the Sec complex compoents in different colors. The map is shown at contour level 0.028, with the exception of the Sec71 part, which is shown at level 0.02. c, Side views of the models for the Sec complex components in a ribbon diagram.