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. Author manuscript; available in PMC: 2019 Jun 30.
Published in final edited form as: Nature. 2018 Dec 31;566(7742):136–139. doi: 10.1038/s41586-018-0856-x

Extended Data Fig. 7 |. Sec63 expression in wild-type and mutant S. cerevisiae cells.

Extended Data Fig. 7 |

The strains used in Fig. 3c were analyzed for expression of FLAG-tagged Sec63. FLAG-tagged wild-type or mutant Sec63 was expressed in sec63Δ S. cerevisiae cells. Equal number of cells were analyzed by SDS-PAGE, followed by immunoblotting with FLAG antibodies. To control for equal loading, the samples were also analyzed with antibodies to phosphoglycerate kinase (PGK). The lanes correspond to expression of the following Sec63 proteins: 1) wild-type Sec63; 2) mutations in interface #3 of Sec63 (Y5A, Y7A, D8A); 3) mutations in interface #2 (Y227A, W242A); 4) mutations in interface #2 (Y227A, W242A, W243A); 5) mutations in interface #1 (T444K, S447K, E482A); 6) empty vector. The experiment was repeated twice.