Extended Data Table 1 |.
Cryo-EM data collection, refinement, and validation statistics.
| Sec Complex (EMDB-0440) (PDB 6ND1) | |
|---|---|
| Data collection and processing | |
| Magnification | 81,000 |
| Voltage (kV) | 300 |
| Electron exposure (e−/Å2) | 54.8 |
| Defocus range (μm) | −1.0 to −2.8 |
| Pixel size (Å) | 1.35 |
| Symmetry imposed | C1 |
| Initial particle images (no.) | 2,280,162 |
| Final particle images (no.) | 91,218 |
| Map resolution (Å) | 4.1 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 3.5 to 6.5 |
| Refinement | |
| Initial model used (PDB code) | |
| Model resolution (Å) | 4.1 |
| FSC threshold | 0.5 |
| Model resolution range (Å) | |
| Map sharpening B factor (Å2) | −180 |
| Model composition | |
| Non-hydrogen atoms | 9189 |
| Protein residues | 1179 |
| Ligands | |
| B factors (Å2) | |
| Protein | 72.61 |
| Ligand | |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.009 |
| Bond angles (°) | 1.254 |
| Validation | |
| MolProbity score | 1.68 |
| Clashscore | 7.55 |
| Poor rotamers (%) | 0.1 |
| Ramachandran plot | |
| Favored (%) | 96.16 |
| Allowed (%) | 3.76 |
| Disallowed (%) | 0.08 |