Figure 4.

Effects of epinastine on the execution of the CR after standard training and extended training. (a,b) Groups of animals were each subjected to a pretest and then four trials × 1 day training (a) or four trials × 3 days training (b). On the next day, crickets in each group were injected with 3 µl of saline or saline containing 2 µM epinastine 30 min before the test. Peppermint odour was used as the CS and vanilla odour was used as the control odour. (c,d) The experiments were replicated with either apple odour or banana odour being used as the CS and the other being used as the control odour (indicated as a:b). Relative preferences for the rewarded odour (PI) before (white box) and 1 day after training (grey box) are shown as box plots. The results of statistical comparison before and after conditioning (WCX test) and between groups (M–W test) are shown as asterisks (*p < 0.05; **p < 0.01; ***p < 0.001; n.s. p > 0.05, with the p-values adjusted by Holm's method).