Newly diagnosed DM is characterized by decreased TLR induced secretion of pro and anti-inflammatory cytokines by peripheral blood leukocytes (PBLs) as determined by ELISA. Box and whisker plots showing the levels of TNF-α (a), IL-6 (b), IL-1β (c) and IL-10 (d) in the supernatants of PBL cultures following TLR2 and 4 stimulation in NGT/control (n = 42), NDD (n = 34) and KDM (n = 53) subjects. Statistical significance was determined by non-parametric Mann–Whitney U test and p < 0.05 was considered significant. NGT – normal glucose tolerance, NDD – newly diagnosed diabetic, KDM – known diabetic subjects.