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. Author manuscript; available in PMC: 2019 Feb 8.
Published in final edited form as: Cytokine. 2015 May 8;76(2):253–259. doi: 10.1016/j.cyto.2015.04.010

Fig. 3.

Fig. 3.

Newly diagnosed DM is characterized by decreased expression of IL-6, TNF-α and CD69 in B cells following TLR stimulation as determined by flowcytometry. Box and whisker plots showing the % of B cells expressing TNF-α (a) and IL-6 (b) in NGT/control (n = 13), NDD (n = 14) and KDM (n = 15) subjects are shown. Histogram analysis showing the downregulation of TNF-α in TLR2 (c) and TLR4 (d) stimulated B cells (shaded area-Isotype control; dotted line-NDD; grey line-NGT and black line-KDM. Box and whisker plots showing the % of B cells expressing CD69 (e) and activated caspase-3 (f) in NGT/control, NDD and KDM subjects are shown. Statistical significance was determined by non-parametric Mann–Whitney U test and p < 0.05 was considered significant. NGT – normal glucose tolerance, NDD – newly diagnosed diabetic, KDM – known diabetic subjects.