Figure 5.

Nuclear PAK4 induces ERα+ breast cancer metastasis to the bone. MCF-7-Luc-F5 cells were infected with lentiviruses carrying either a control vector (Con), PAK4 expression construct (PAK4-Lv), control shRNA (shCtrl), or PAK4 shRNA (shPAK4), and were injected into tail vein of 6-week-old female SCID mice. a, b Representative in vivo BLI images of animals at the indicated time points are shown. Normalized BLI signals of metastases of mice injected intravenously with the indicated transfected cells. c, d Representative BLI images of eight animals after tail vein injection of Con and PAK4-overexpressing MCF-7-Luc-F5 cells at the indicated time points. e, f The effect of PAK4 on seeding bone metastases in mice intravenously injected with breast cancer cells. BLI and X-ray images and BLI of bone metastatic tissues from mice injected with MCF-7-Luc-F5 breast cancer cells transfected with a PAK4 overexpression vector. Bone sections from PAK4-overexpressing mice were stained with H&E. g, h Representative BLI images showed tumor growth primarily within the lung and bone metastases from Con group and PAK4-Lv group