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. 2019 Feb 1;10:57. doi: 10.3389/fpls.2019.00057

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Copyright © 2019 Jones, Morohashi, Grotewold and Harmer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chromatin Immunoprecipitation assays to evaluate JMJD5-GFP association with circadian gene loci. Enrichment of genomic DNA fragments from the CCA1 (A), PRR7 (B) and PRR9 (C) promoters following chromatin immunoprecipitation with a GFP antibody. Plants were grown for 10 days at 22°C under 12:12 light:dark cycles and harvested at either ZT0 or ZT12. Presented data represent fold-increases compared to enrichment at the EF1a promoter and are the mean of four biological independent replicates. Error bars represent SEM.