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. 2019 Feb 1;10:57. doi: 10.3389/fpls.2019.00057

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Copyright © 2019 Jones, Morohashi, Grotewold and Harmer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chromatin Immunoprecipitation assays to evaluate histone H3K36 methylation at circadian gene loci. Enrichment of genomic DNA fragments from the CCA1 (A), PRR7 (B) and PRR9 (C) promoters following chromatin immunoprecipitation with H3K36me1, H3K36me2 or H3K36me3 antibodies. Plants were grown for 10 days at 22°C under 12:12 light:dark cycles and harvested at either ZT0 or ZT12. Data from four independent experiments is shown and represents fold-increases compared to input. Error bars show standard deviation.