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. 2019 Feb 1;7:39. doi: 10.3389/fchem.2019.00039

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Copyright © 2019 Wang, Qiu, Liu, Jayawardhana, Yue, Daghlas, Bowers, Datta and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Nanoformulation of Cage 1 using fluorescein (2) and MPEG_5k-PGA₅₀ (3). (A) Quenching of fluorescence emission of fluorescein by titration with 1 (6 μM) and the Scatchard plot extracted from fluorescence titration to determine K_d; (B) Changes of the absorption spectra of the mixture of 1 and 2 in a different molar ratio (0–1.0) and the Job plot extracted from titration to determine the binding ratio of 1 and 2.; (C) A cryo-EM image (Scale bar: 20 nm) (D) DLS analysis of size distribution; (E) Zeta-potential analysis; (F) UV-vis spectra of fluorescein and nMOC in PBS ([2] = 20 μM).