Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 1;10:22. doi: 10.3389/fendo.2019.00022

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Husić, Barsyte-Lovejoy and Lovejoy.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

FITC-K37-mTCAP-1 co-localizes with LPHN1 expression. I: (A–E) HEK-WT cells treated with FITC-K37-mTCAP-1. (F–J) HEK-Puro cells treated with FITC-K37-mTCAP-1. (K–O) HEK-LPHN1-S cells treated with FITC-K37-mTCAP-1. Arrows indicate regions of FITC-K37-mTCAP-1 and LPHN1-S co-localization. (A,F,K) DIC. (B,G,L) FITC-K37-mTCAP-1. (C,H,M) FLAG (LPHN1-S) expression. (D,I,N) FITC-K37-mTCAP-1 and FLAG (LPHN1-S) fluorescence channel overlay images. (E,J,O) Merged image of all channels. A low level of green fluorescence is seen in HEK-WT and HEK-Puro cells, indicating some uptake of FITC-K37-mTCAP-1. However, HEK-LPHN1-S cells show a much brighter green fluorescence signal, indicating a higher uptake of FITC-K37-mTCAP-1. Scale bar in all images is 25 μm. (P) Average number of green pixels per cell with an intensity ranging from 20 to 255 in HEK-WT, HEK-Puro, and HEK-LPHN1-S cells treated with FITC-K37-mTCAP-1. Pixel intensity range is indicated above graph. HEK-LPHN1-S cells have a significantly higher number of green pixels with intensities of 20–255 per cell, indicating a higher degree of FITC-K37-mTCAP-1 uptake compared to HEK-WT and HEK-Puro cells. No significant differences between HEK-WT and HEK-Puro cells were observed. (Mean ± SEM; n = 5; ^****p < 0.0001; one-way ANOVA) II: (A–J) Confocal images of HEK-LPHN1-S cells. (A,F) DIC. (B,G) FITC-K37-mTCAP-1. (C,H) LPHN1-S. (D,I) Merged images of B–C and G–H, respectively. (E,J) Merged images of A–C and F–H, respectively. Scale bar in A–E is 20 μm and in F–J is 6 μm. Regions of FITC-K37-mTCAP-1 and LPHN1-S co-localization on the cell membrane were predominant (arrows labeled 1); however, areas of only FITC-K37-mTCAP-1 localization (arrows labeled 2) or of only LPHN1-S localization (arrows labeled 3) were also seen present in HEK293-LPHN1-S cells.