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Expression plasmid with all mup genes needed for conversion of PA-B to PA-A. (A) Plasmid map. (B) Functional bioassays for each gene in the constructed plasmids (see Table 1) using B. subtilis; the best performing IPTG induction condition is shown, which was 0.5 mM IPTG for ΔmupM [pJC132] and ΔmupN [pJC132] and 0 mM IPTG for all other samples; n = 3, error bars are standard deviation. For ΔmupN the strain with pJH10 gave so little activity the column is not visible.
