Fig. 7.

Deletion of PPAD decreases secreted Rgp enzymatic activity and increases Rgp and Kgp enzymatic activity in biofilms. Rgp enzymatic activity in a planktonic cultures and c biofilm lysates, and Kgp enzymatic activity in b planktonic culture and d biofilm lysates was measured using a colorimetric assay. a Less Rgp activity was measured in the supernatants of Δ8820 cultures. b There were no differences in Kgp activity between 381 and Δ8820. Both c Rgp and d Kgp enzymatic activities were greater in Δ8820 biofilm lysates than 381 biofilm lysates. Data are representative of three independent experiments showing similar results (n = 3). Error bars represent the standard deviation of technical replicates. The data were analyzed using the Student’s two-tailed t-test. *p < 0.05