Table 2.
Primers and probes used for qPCR. Sequence of the primers and their corresponding probes. Probe numbers refer to numbers in the universal probe library (Roche).
| Target gene | Primer sequence 5′-3′ | Universal probe/SYBR green |
|---|---|---|
| BiP | fw: GCCAACTGTAACAATCAAGGTCT rev: TGACTTCAATCTGGGGAACTC |
#15 |
| CHOP | fw: CCACCACACCTGAAAGCAG rev: TCCTCATACCAGGCTTCCA |
#33 |
| XBP1s | fw: TCCGCAGCAGGTGCAG rev: CCAACTTGTCCAGAATGCCC |
SYBR green |
| XBP1u | fw: GCAGCACTCAGACTATGTG rev: CCAACTTGTCCAGAATGCCC |
SYBR green |
| ERdj4 (DNAJB9) | fw: TGTGTGTAGTCACTCTTTTGCACT rev: TTGCACATAATAAGGTTACACAGAAA |
#71 |
| GRASP55 | fw: CATGTGCTGGAAGTGGAATC rev: GCTGAACAGGTCTTCAGACTCA |
#3 |
| GRASP65 | fw: ACACGTGTGGCATGTGCT rev: GAGCCAACTATGTAGTCTGTGTAAGG |
#62 |
| EEF1A1 | fw: ACACGTAGATTCCGGCAAGT rev: AGGAGCCCTTTCCCATCTC |
#31 |