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. 2019 Feb 7;10:636. doi: 10.1038/s41467-019-08481-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — USP11 suppresses cancer cell biology in a PTEN-dependent manner. a Growth curves of HAP1 cells knocked out (KO) for USP11 using CRISPR/Cas9 technology expressing wild-type (WT) or catalytically inactive C318S (CS) mutant of USP11. n = 3. **p < 0.01, ***p < 0.001, Cont. vs. USP11^WT; ^$$p < 0.01, ^$$$p < 0.001, USP11^WT vs. USP11^CS; ^#p < 0.05, ^##p < 0.01, Cont. vs. USP11^CS. b Cell-invasion assays of HAP1 cells KO for USP11 expressing WT or CS mutant of USP11 in the presence of mitomycin C (5 μg ml⁻¹) (top). The number of invaded cells per field was quantified (bottom). n = 3. c The rates of glucose uptake, lactate production, and glutamine consumption of HAP1 cells KO for USP11 expressing WT or CS mutant of USP11 were measured and normalized to cell number. n = 3. d Growth curves of PC3 PTEN^WT and PC3 PTEN^-/- cells expressing USP11. n = 3. *p < 0.05, **p < 0.01, PTEN^WT; Cont. vs. PTEN^WT; USP11; n.s., non-significant, PTEN^-/-;Cont. vs. PTEN^-/-;USP11. e Cell-invasion assays of PC3 PTEN^WT and PC3 PTEN^-/- cells ectopically expressing USP11 in the presence of mitomycin C (5 μg ml⁻¹) (top). The number of invaded cells per field was quantified (bottom). n = 3. f The rates of glucose uptake, lactate production, and glutamine consumption of PC3 PTEN^WT and PC3 PTEN^-/- cells ectopically expressing USP11 were measured and normalized to cell number. n = 3. g Growth curves of PC3 PTEN^WT and PC3 PTEN^-/- cells expressing USP11 shRNA. n = 3. *p < 0.05, ***p < 0.001, PTEN^WT;shCont. vs. PTEN^WT;shUSP11; n.s., non-significant, ^$$$p < 0.001, PTEN^-/-;shCont. vs. PTEN^-/-;shUSP11. h Cell-invasion assays of PC3 PTEN^WT and PC3 PTEN^-/- cells expressing USP11 shRNA in the presence of mitomycin C (5 μg ml⁻¹) (top). The number of invaded cells per field was quantified (bottom). n = 3. i The rates of glucose uptake, lactate production, and glutamine consumption of PC3 PTEN^WT and PC3 PTEN^-/- cells expressing USP11 shRNA were measured and normalized to cell number. n = 3. Scale bars, 100μm. Error bars represent ± SEM. p Value was determined by Student’s t test (n.s., non-significant; *p < 0.05, **p < 0.01, ***p < 0.001)