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. 2019 Feb 7;10:636. doi: 10.1038/s41467-019-08481-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — A PTEN-PI3K-FOXO-USP11 auto-regulatory feedforward mechanism. a, b Lysates and total RNAs from PTEN^+/+, PTEN^+/-, and PTEN^-/- HCT116 cells were subjected to immunoblotting (IB) (a) and RT-qPCR (b). n = 3, p value was determined by ANOVA. (c) Luciferase reporter analysis of the USP11 promoter in PTEN^+/+, PTEN^+/-, and PTEN^-/- HCT116 cells. n = 3, p value was determined by ANOVA. d Chromatin levels of FOXO1 at proximal promoters of human USP11 were compared between PTEN^+/+ and PTEN^-/- HCT116 cells by quantitative ChIP assays. Enrichment of FOXO1 was analyzed with respect to the input control (before IP) and normalized to IgG control. TSS, transcription start site. n = 3. e Lysates and total RNAs from anterior prostates of wild-type (Pten^pc+/+) and Probasin-Cre4;Pten^loxP/loxP (Pten^pc-/-) mice at 11 weeks of age (n = 4) were subjected to IB (top) and RT-qPCR (bottom). f Lysates and total RNAs from PTEN^+/+, PTEN^+/-, and PTEN^-/- HCT116 cells treated with 500 nM BKM120 for 24 h were subjected to IB (top) and RT-qPCR (bottom). n = 3. g Lysates and total RNAs from PTEN^+/+, PTEN^+/-, and PTEN^-/- HCT116 cells treated with 500 nM VO-OHpic for 8 h were subjected to IB (top) and RT-qPCR (bottom). n = 3. h Lysates from PTEN^+/+ HCT116 cells treated with 500 nM BKM120 for 24 h together with the absence or presence of MG132 (10 μM, 8 h before harvesting) were subjected to IB. i Lysates from p85^+/+ and p85^-/- MEFs treated with 10 μM MG132 for 8 h were subjected to IB. j Lysates from PTEN^-/- HCT116 cells transfected with wild-type (WT) or phosphatase-inactive PTEN^C124S and PTEN^G129E subjected to IB. k A model for a PTEN-PI3K-FOXO-USP11 auto-regulatory feedforward mechanism. Error bars represent ± SEM. p Value was determined by Student’s t test (n.s. non-significant; *p < 0.05, **p < 0.01, ***p < 0.001). ChIP, chromatin immunoprecipitation; IP, immunoprecipitation; MEFs, mouse embryonic fibroblasts; RT-qPCR, quantitative reverse transcription PCR