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. 2019 Feb 7;10:627. doi: 10.1038/s41467-019-08543-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — 24-nt phasiRNAs with 12-nt phasing in tomato. a 24-nt phasiRNA accumulation in different tissues of tomato, with each row denoting a 24-PHAS locus. b A representative locus generating 24-nt phasiRNAs in tomato. Distribution of sRNAs (above) and deduced phasing score (below) are viewed along the coordinates of the PHAS locus, with phasiRNA distribution in registers displayed in a radar plot on the left. c A hypothesis for the generation of 24-nt phasiRNAs with a 12-nt phasing pattern. Cis activity of either strand of a 24-nt phasiRNA duplex could trigger secondary phasing with a 12-nt shift to the primary 24-nt phase. The combination of the primary and secondary phases leads to the formation of the 12-nt phase pattern