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. 2019 Feb 7;9:1590. doi: 10.1038/s41598-018-38363-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Live imaging of apoptotic photoreceptors in vivo by retinal imaging. (a) Representative fluorescence images of photoreceptors and fundus images of p25 RCS and WT rats 24 hours after PSVue-550 or HBSS solvent eyedrop application as indicated. (b) Quantification of fluorescence intensity after background subtraction in areas indicated by dashed lines. *P < 0.05 by Student’s t-test. (c) Photoreceptor fluorescence and (d) quantification as in b of fluorescence intensity maxima. *P < 0.05 Student’s t-test. (e) Photoreceptor fluorescence and (f) quantification of areas of fluorescent intensities specific to retinal quadrants. *P < 0.05; two-way ANOVA and Tukey post-hoc test. (b,d,f) All bars show mean ± SEM, n = 3 animals per group.