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. 2019 Feb 7;9:1584. doi: 10.1038/s41598-019-38505-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analyses on exogenous oligonucleotide. (a) Arbitrary stability value and Acc.SD. of exogenous oligonucleotide (cel-39, UniSp2, UniSp4 and UniSp6) calculated by NormFinder. Spike-ins are ranked as stable (black) and unstable (white). The analysis of the expression stability was performed comparing free-circulating miRNAs from sedentary and trained subjects. (b) CV% of all analyzed miRNAs normalized on mean-spike-in (average of cel-39, UniSp2 and UniSp4) and on cel-39, UniSp2, UniSp4 and UniSp6, singularly. (c) Percentage of the frequency distribution of CV of all analyzed miRNAs normalized as in (b). Median of the CV% distribution is indicated for each graph.