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. 2019 Feb 7;9:1587. doi: 10.1038/s41598-018-38182-2

Figure 4.

Figure 4

Uptake of 2 and 3 into rat kidney slices (A–C) or into MDCK II cells stably expressing OAT1 or OAT3 (D–F). Female EHBRs were orally treated with GA (200 mg/kg), and their plasma samples were collected 24 h after the treatment. The concentrations of compounds 2, 3, and GA in the plasma samples of EHBRs were 7.8, 11.5, and 7.0 µM, respectively. Kidneys were collected from normal SD rats, and the kidney slices were incubated with the plasma samples of EHBRs at 37 °C or 4 °C for 2 h (A–C). MDCK II cells stably expressing OAT1 or OAT3 were incubated with the plasma samples of EHBRs at 37 °C for 15 min (D–F). Then, the concentrations of compounds 2, 3, and GA in kidney slices or the cells were measured by LC-MS/MS. Data are expressed as mean ± S.E. (n = 3 for (C, D), and (E); n = 6 for (A, B), and F). *P < 0.05 and **P < 0.01 vs 4 °C group by Student’s t-test for (A–C), and vs mock cells by Dunnett’s multiple t-test for (D–F).